BIO 5300 Recombinant DNA Technology

Students isolate plasmid and chromosomal DNA from multiple sources, perform and optimize PCR amplification, clone sequences into plasmid vectors, perform real time qPCR to quantitate microbial populations in a mixed sample, and carry out CRISPR gene editing in yeast. Related topics include choice and construction of vectors and use of PCR technology for various molecular applications. Students will also research candidate genes they hypothesize to affect aging and will then test their hypothesis by cloning the gene into an appropriate vector, introducing the recombinant vector into yeast, and assaying effects on aging. Lecture and lab. Students will be charged an additional Biology Lab Fee when enrolling in this course. Prerequisite(s): Matriculation in the M.S. Biotechnology program.
Credits: 4.0