BIO 4300 Biotechnology:DNA

Techniques of DNA purification and quantification. Students analyze DNA by Southern transfer, prepare plasmid and phage vectors and clone sequences into those vectors. Antibiotic selection, filter screening and hybridization analysis of cloned libraries are performed. Related topics include prokaryotic and eukaryotic gene structure, choice and construction of vectors and applications of genetic engineering. Lecture and lab. Restricted to Biotechnology majors; Biology majors may register with permission of instructor. Students will be charged an additional Biology Lab Fee when enrolling in this course.
Credits: 4.0