BIO 6320 Biotechnology: Protein

Students are introduced to methods of enzyme extraction, isolation and enzymatic assays. HPLC is employed for protein purification. Protein gel electrophoreses, both one- and two-dimensional, are performed. Western blotting and image analysis techniques are used. Related topics to be covered include posttranslational modifications, biochemistry of proteins, related isolation techniques, radiolabeling of peptides, subcellular localization and protein activity. Lecture and lab. Students will be charged an additional Biology Lab Fee when enrolling in this course. Prerequisite(s): BIO 5240 and BIO 5300 or permission of the instructor
Credits: 4.0